Fig. 1

Experimental design. Groups of adult male zebrafish are maintained in normoxia (20–21 kPa, n = 20) or hypoxia (11–12 kPa, n = 20) for 14 days. Five males from each treatment were then used to create F1 progeny, crossing the males to unexposed females (n = 5 families/treatment), with half of the sperm used for whole genome bisulfite sequencing, to assess differential methylation (n = 3 per treatment). At 20–21 days post fertilization, offspring undergo acute hypoxia tolerance (6–8 offspring/family) assays and n = 3 offspring/treatment are used for differential gene expression analysis (RNA-Seq)