Skip to main content
Fig. 2 | BMC Biology

Fig. 2

From: Lipophorin receptors regulate mushroom body development and complex behaviors in Drosophila

Fig. 2

Decreasing expression of LpR1 or LpR2 in MB results in anatomical defects. a Scheme of the normal organization of the MB in the adult fly brain. MB subregions are identified. b–g Representative images of phenotypes found in adult MB. c, e, f, and g are images from c309,eGFP/ + ;LpR1DF/ + brains. d is an example from c309,eGFP/ + ;LpR2DF/ + brain; in green is shown GFP expression in MB neurons (c309,eGFP), in magenta is presented FasII staining to visualize the MB structure. Except f, all microphotographs correspond to a limited number of optical sections that help observe the phenotypes in each image. Empty arrowheads point to normal structure in MB lobes; full arrowheads show alterations. Scale bar: 20 µm. h Percentage of fly brains from each genotype exhibiting identified phenotypes; data is presented as strain (number of brains, number of independent experiments): c309,eGFP control animals (40, 4); c309,eGFP/ + ;LpR1DF/ + (41, 4); c309,eGFP/ + ;LpR1DF/LpR1DF (35, 2); c309,eGFP/ + ;LpR2DF/ + (46, 4); c309,eGFP/ + ;LpR2DF/LpR2DF (31, 2). Fisher test P = 6.46 × 10−9. i Distance between β lobes in each side of the fly brain in the different genotypes. Data expressed as mean ± SEM from the number of brains identified in h; one-way ANOVA, Kruskal Wallis post-test, p = 0.1486, “ns,” not significant. j Percentage of brains exhibiting phenotypes in flies expressing RNAi for LpR1 or LpR2 in MB neurons. Fisher test P = 1.02 × 10−13. k Distance between β lobes in knockdown strains. Data in j and k from c309,eGFP control flies (26, 4); RNAi-LpR1/ + control flies (15, 1); c309,eGFP/ + ;RNAi-LpR1/ + (21, 4); RNAi-LpR2/ + control flies (18, 1); c309,eGFP/ + ;RNAi-LpR2/ + (27, 4). One-way ANOVA, followed by Kruskal Wallis test p < 0.0001; * and ****, p = 0.0206 and p < 0.0001 respectively; “ns,” not significant

Back to article page