Fig. 4

Mushroom body neurons respond to Reelin. a Representative images of MB neurons in primary cultures of the c309,eGFP strain, after Mock or Reelin treatment. 13 nM Reelin and 30 nM Reelin treatment correspond, respectively, to 20 and 50% dilution of Reelin conditioned medium in the cell culture. Mock 1 and Mock 2 treatments are the experimental controls of 13 and 30 nM Reelin, respectively. MB neurons are identified by GFP expression. Black bars in the lower right corner represent 5 µm. b Sholl profile of MB neurons after different treatments. Data are expressed as mean ± SEM; two-way ANOVA, p < 0.0001 for each factor. “*” indicates a significant difference (p < 0.05) between the 13 nM Reelin and the corresponding Mock 1 treatment, at a given distance from the soma; “ + ”, significant difference (p < 0.05) between 30 nM Reelin and Mock 2 treatment, at the same distance from the soma. “#”, (p < 0.05) between effects at 13 and 30 nM Reelin concentrations at the same distance from the soma. c Maximum length, defined as the greater distance reached for a cell’s neurite with respect to its soma, recorded in MB neurons under the different treatments. Data expressed as mean ± SEM; one-way ANOVA, followed by Kruskal Wallis post hoc test, p < 0.0001. *** indicates p < 0.0005. d Critical value, the number of neurites found in the radius with most neurites, in MB neurons under each treatment. Data is mean ± SEM; one-way ANOVA and Kruskal Wallis post-test, p < 0.0001. *, ****, are p < 0.05, p < 0.0001. “ns” not significant. Data from n = 20 coverslips in four independent experiments, 15–20 cells in each “n”