Fig. 7

Reelin effects on mushroom body neurons depend on Dab. a–c Reelin does not increase neurite complexity in neurons from Dab mutant flies. a Sholl profile of cultured MB neurons from Dab (c309,eGFP/ + ;Dab¹/ +) mutant flies and their controls (c309,eGFP), after Mock or Reelin treatment. Two-way ANOVA shows that treatment and genotype factors and their interaction play a role in results obtained (p < 0.0001 for all). “#”, significant differences (p < 0.05) after Reelin treatment between the genetic control and all other experimental groups. There were no significant differences between all other conditions. b Maximum length and c critical value in mutant and control flies, after mock or Reelin treatment. In b, c, two-way ANOVA shows that treatment and genotype factors and the interaction between factors play a role in the results (p < 0.0001 for each analysis); ****, indicates p < 0.0001 between conditions. d–f Reelin does not affect neurite complexity in knockdown animals for Dab. d Sholl profile, e maximum length, and f critical value measured in flies expressing an RNAi for Dab only in MB neurons (c309,eGFP/ + ;RNAi-Dab/ +), as compared to controls (c309,eGFP), after Mock or Reelin treatment. In d, two-way ANOVA shows that treatment and genotype factors and their interaction play a role in results obtained for each data set (p < 0.0001 for all). “#”, significant differences (p < 0.05) between control and Dab knockdown after Reelin treatment. “*”, significant differences between knockdown and control, after Mock treatment. “ + ”, significant differences (p < 0.05) between mock and Reelin treatment on the knockdown fly strain. In e, f, two-way ANOVA indicates that genotype and treatment factors, and their interaction play a role in results (p < 0.0001, p < 0.01, and p < 0.05 respectively for each condition). *** and ****, p < 0.005 and p < 0.0001; “ns”, not significant. Data in a–f, from n = 12 coverslips 4–20 cells studied in each coverslip, three independent experiments