Fig. 9
From: Lipophorin receptors regulate mushroom body development and complex behaviors in Drosophila
Dab interact with LpR1 for the correct establishment of the MB structure. a Representative images of the phenotypes observed in MB of flies lacking one copy of Dab (c309,eGFP/ + ;Dab1/ +) or that express RNAi against Dab in MB neurons (c309,eGFP/ + ; RNAi-Dab/ +). Green, GFP; Magenta, Fas II immunostaining. Empty arrowheads show normal MB structure; full arrowheads indicate structural alterations. White bar: 20 µm. b Distribution of phenotypes observed in control c309,eGFP (n = 36 brains, 4 experiments) and haploinsufficient mutant c309,eGFP/ + ;Dab1/ + (n = 34 brains, 4 experiments) animals. Fisher test, p = 3.44 × 10−4. c Distance between β lobes in brains from Dab haploinsufficient mutants as compared to controls; t-test, shows significant differences (*p = 0.0107); data in c309,eGFP from n = 36 brains, 4 experiments, and c309,eGFP/ + ;Dab1/ + from n = 40 brains, 4 experiments. d Relative percentage for phenotypes observed when RNAi against Dab transcript is expressed in MB neurons. Fisher test, p = 2.2 × 10−16 in c309,eGFP (n = 26 brains, 3 experiments,), RNAi-Dab/ + (n = 18 brains, 1 experiments) and in c309,eGFP/RNAi-Dab (n = 34 brains, 3 experiments) flies. e Distance between β lobes in flies expressing RNAi for Dab in MB neurons as compared to control animals; one-way ANOVA (p < 0.0001) followed by Kruskal Wallis. ****, p < 0.0001. Data from c309,eGFP (n = 26 brains, 3 experiments), RNAi-Dab/ + (n = 18 brains, 1 experiments) and in c309,eGFP/RNAi-Dab (n = 34 brains, 3 experiments). f Relative percentage of brains with MB defects in double LpR1 and Dab heterozygous mutants (LpR1 CRIMIC/Dab1) as compared with haploinsufficient animals for each gene. Data obtained from Dab1/ + animals in n = 20 brains, 2 experiments; LpR1 CRIMIC/ + animals in n = 57 brains, 4 experiments (same brains used in the Additional file 1: Fig. S4); and LpR1 CRIMIC/Dab1 in n = 28 brains, 2 experiments. Fisher test, p = 3.7 × 10−16. g Distance between β lobes in double LpR1 CRIMIC/Dab1 mutant animals as compared to heterozygous mutants in each gene. Data obtained from Dab1/ + animals in n = 20 brains, 2 experiments; LpR1 CRIMIC/ + animals in n = 57 brains, 4 experiments (same brains used in the Additional file 1: Fig. S4); and LpR1 CRIMIC/Dab1 in n = 28 brains, 2 experiments. One-way ANOVA (p = 0.0002) followed by Kruskal Wallis. * and ** means p = 0.0156 and p = 0.0002 respectively. “ns” means not statically significant