Fig. 5

Homoeolog expression changes, cis- and trans-regulations, and their correlation with K_a/K_s. A The extent of homoeolog expression bias (HEB) was observed in different developmental stages and tissues. Different gene numbers (705, 762, 2810, 5807, 3039, and 9707 in the blastula, gastrula, segmentation, hatching periods, and liver and barbel tissues, respectively) are assessed in this graph. The density distribution of log₂ (R/C) values was determined in the inbred parents (in silico hybrid), F₁, and F₂₂. Dotted lines represent the average log₂ (R/C) values in each sample. B Numbers and percentages of cis- and trans-regulatory genes in different developmental stages and tissues. C Distribution of K_a/K_s values of OGPs in the “cis-only”, “trans-only”, and total genes (all) of F₁. The red dots in boxes represent the mean values. D Correlation analyses of potential DNA MRGs and “cis-only” genes in F₁ and F₂₂. The grey represents “no cis only” genes. The black represents “cis only” genes. The black represents “ambiguous” genes