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Fig. 2 | BMC Biology

Fig. 2

From: Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada

Fig. 2

Super-resolution imaging of Giardia lamblia peripheral vesicles with stimulated emission depletion (STED). A Giardia trophozoites loaded with 10-kDa Dextran-AlexaFluor 594 were imaged using confocal and STED microscopy. Dorsal (upper row) and ventral (lower row) regions are represented. In contrast to confocal imaging, STED microscopy allows to separate individual organelles and to visualize different endocytic compartment morphologies. ROI, region of interest. B Organelle segmentation with ilastik distinguishes three dextran-labelled PV categories. C PV areas were calculated post-segmentation on maximum projections of the dorsal regions of 15 cells, using ilastik. Spherical PVs (green, N = 1684) have an average area of 0.0205 ± 0.0169 μm2 with a 95% confidence interval between 0.0197 and 0.0213, tubular PVs (blue, N = 835) have an average area of 0.0453 ± 0.0278 μm2 with a 95% confidence interval between 0.0435 and 0.0472 and polymorphic PVs (magenta, N = 400) have an average area of 0.0981 ± 0.0429 μm2 with a 95% confidence interval between 0.0939 and 0.102. The differences in the area are statistically significant (ANOVA; p-value < 0.0001). Scale bars: A 5 μm and 1 μm (ROIs), B 5 μm

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