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Fig. 3 | BMC Biology

Fig. 3

From: Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada

Fig. 3

Super-resolution imaging of Giardia lamblia PVs by stochastic object reconstruction microscopy (STORM). A Widefield microscopy-based imaging of a Giardia trophozoite loaded with 10-kDa Dextran-Alexa Fluor 647. B Reconstruction of single-molecule events using the Fiji plugin Thunderstorm. As with STED imaging, different PV morphologies are observed. C PVs were segmented (N = 10 cells) with the help of ilastik, and volumes were calculated and plotted in (D). Spherical PVs (green, N = 1989) present an average volume of 0.00507 ± 0.00336 μm3 with a 95% confidence interval between 0.00492 and 0.00522 μm3, tubular PVs (blue, N = 838) present an average volume of 0.0103 ± 0.00925 μm3 with a 95% confidence interval between 0.00967 and 0.0109 μm3 and polymorphic PVs (magenta, N = 1494) present an average volume of 0.0227 ± 0.0214 μm3 with a 95% confidence interval between 0.0216 and 0.0238 μm3. The differences in the area are statistically significant (ANOVA; p-value < 0.0001). Based on these and previously shown data, the renaming of PVs to peripheral endocytic compartments (PECs) is proposed. Scale bars: 5 μm and 1 μm (ROI)

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