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Fig. 5 | BMC Biology

Fig. 5

From: Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada

Fig. 5

Super-resolution imaging of S. vortens endocytic compartments with STED and transmission electron microscopy (TEM). A Following incubation with dextran-TexasRed for 30 min, S. vortens display elongated endocytic compartments with an average diameter of 468 ± 206 nm with a 95% confidence interval between 421 and 515 nm (upper violin plot, N = 10 cells). B TEM imaging detects several endosome-like vacuoles (V) throughout the cell, with an average diameter of 844 ± 335 nm with a 95% confidence interval between 763 and 905 nm (lower violin plot). Measurements were done manually. The endoplasmic reticulum (ER) and a nucleus (N) are also highlighted in the images. Scale bars: 5 μm (full field of view) and 1 μm (ROIs)

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