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Fig. 6 | BMC Biology

Fig. 6

From: Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada

Fig. 6

Super-resolution imaging of T. foetus endocytic compartments with STED and TEM. A 2D-STED analysis of T. foetus cells loaded with 10-kDa Dextran-Alexa Fluor 594 illuminates endosomes with globular structures at an average diameter of 517 ± 251 nm with a 95% confidence interval between 455 and 580 nm (upper violin plot, N = 10 cells). B TEM investigation of T. foetus cells reveals two kinds of endosome-like vesicles. Low electron-density vesicles found both at the cell periphery and centre are deemed vacuoles (V) with an average diameter of 246 ± 100 nm (N = 153) with a 95% confidence interval of 230 and 262 nm. Vesicles of higher electron density and with noticeable content are digestive vacuoles (DVs). DVs are larger than vacuoles, with an average diameter of 764 ± 203 nm (N = 50) with a 95% confidence interval of 707 and 822 nm (lower violin plot). Scale bars: 5 μm (full field of view) and 1 μm (ROIs)

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BMC Biology

ISSN: 1741-7007

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