Fig. 8

Homology search for bona fide CHC and CLC reveals the key importance of CHC and patchy conservation of CLC. A CHC and CLC homology searches demonstrate the conservation of CHC orthologs in all analysed lineages. However, several lineages appeared to have lost a readily detectable bona fide CLC including all selected Fornicata, certain Chromerids [77], some ciliates [78] and members of the Hemimastigophora. B Ab initio in silico protein modelling of G. lamblia ACLC (formerly GlCLC/Gl4529), T. brucei CLC, T. foetus CLC, M. exilis CLC, D. discoideum and H. sapiens CLC sequences using AlphaFold, the current standard in in silico protein structure modelling. TM-align and RMSD values were calculated showing a close structure analogy between the predicted structures (table)