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Fig. 6 | BMC Biology

Fig. 6

From: Epigenetic machinery is functionally conserved in cephalopods

Fig. 6

DNA methylation and histone marks are present in squid, and bobtail squid.A Slot blot detecting 5mC on gDNA extracted from arm (2–3 cm of Right Arm 2), brain (optical lobe), gills of one representative animal of D. pealeii and E. berryi, the analogous tissues of O. bimaculoides adults and a 30 dpf hatchling, one representative male mouse adult liver and one representative pool of 5 dpf larvae of zebrafish. Water was used as negative control. B Quantification of 5-mC measured by slot blot was normalized to double-stranded DNA (dsDNA), and each sample was normalized to zebrafish larvae as control. Each dot represents one biological replicate, bars represent the mean among replicates, and error bars represent standard deviations. C Western blot of histone H3 and modifications on an arm of D. pealeii and E. berryi and quiescent adult liver from a male mouse. D Quantification of each sample measured by Western blot was normalized to histone H3. Each dot represents one biological replicate, bars represent the mean among replicates, and error bars represent standard deviations

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