Fig. 6

H3.3 enhancer regulation by ARID1A, CHD4, and ZMYND8. A Heatmap of chromatin features at 15,925 active typical enhancers and 1374 distal super-enhancer constituents (H3K27ac peaks co-marked by ATAC) segregated by ARID1A ± CHD4 ± ZMYND8 binding. Enhancers are centered on the H3K27ac peak, and signal is displayed as indicated for the flanking 5 kb in either direction. B ZMYND8 binding detection at ARID1A-bound typical and super-enhancers with or without CHD4 co-binding. Statistic is two-tailed Fisher’s exact test. C Association between ARID1A+CHD4+ZMYND8 co-binding at enhancers and presence of H3.3. H3.3+ super-enhancers show the most frequent co-binding. Statistic is two-tailed Fisher’s exact test. D Chromatin features at active super-enhancer constituents segregated by ARID1A loss-driven H3K27-acetylation dynamics: hyperacetylated, de-acetylated, or stably acetylated. Left, average ChIP-seq signal density histograms across enhancer classes. Right, violin plots quantifying signal (ChIP/input fold-enrichment) across enhancer classes. Statistic is two-tailed, unpaired Wilcoxon’s test