Fig. 4
From: Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity
Glucan biosynthesis in yeast strains expressing a single starch synthase (SS) and BE2. Upper panels: Glucan content of yeasts expressing the indicated SS and BE2 grown as in Fig. 1B (means ± S.D. from 4 replicate cultures, except for SS2-B [n = 3], SS2-E [n = 3] and SS4-C [n = 5]). Red points depict the individual replicates. The anticipated expression strengths and the estimated molar ratio of the SS to BE2 protein molecules are given on top. Numerical data are provided in Additional file 4, sheet 1. N.d., not determined; WW, wet weight. Middle panels: Iodine staining of cell patches grown for 24 h on galactose-containing plates. Glucose-grown controls are presented in Additional file 1: Fig. S11. Lower panels: Iodine staining of the cell cultures used for glucan extraction. The size bar applies to all images