Fig. 6
From: Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity
Relative protein abundances of BE2 and SS proteins determined by parallel reaction monitoring (targeted proteomics) at the 3-h time point. Quantification used 2–3 surrogate peptides for each protein and isotopically labelled standard peptides for normalization. Protein abundances (means ± S.D., n = 3 replicate cultures; red points show the data from each replicate) are expressed relative to strain 28, which expresses all SS and BE proteins controlled by the pGAL1 promoter [25]. Data was partly replotted in the individual panels for ease of comparison. In the wild-type (WT) samples, the signals of all SS and BE2 surrogate peptides were below the lower limit of quantitation (LLOQ), as expected from the absence of the proteins. Numerical data is provided in Additional file 6, sheet 7