Fig. 7

The genetic divergence of PbCAMTA3 and PbTIC55 between the European and Asian pear populations. A, B Genotypes of “Chr13_5783950” in PbCAMTA3 (A) and “Chr13_5786061” in PbCAMTA3 (B) in cultivated Asian and cultivated European pears showing correlations with relative fruit firmness. C The gene structure of PbCAMTA3 (top) and putative functional amino acid domains in the PbCAMTA3 protein (bottom). D Gene structure of PbTIC55 in Chinese white pear (Pyrus × bretschneideri). E The fruit firmness of seven Asian and six European pear accessions that were used for PCR amplification. F Sequence alignments of sequences from the first exon of the PbTIC55 gene from seven Asian and six European pear accessions showing the 12-bp tandem duplication that is unique to Asian pears. G The relative expression levels of PbTIC55 in nine cultivated Asian pears and six cultivated European pears determined by quantitative real-time PCR analysis (qRT-PCR). H Expression profiles of PbTIC55 during fruit development starting at 15 days after fertilization (DAF) in four cultivated pear accessions, including three Asian pears (“Housi,” “Yali,” “Dangshansuli”) and one European pear (“Starkrimson”). Relative gene expression was determined using reads per kilobase of transcript per million (RPKM) mapped reads values