Fig. 1

AF1_FFF mice exhibit defect in embryo implantation and uterine decidualization. A Domain structure of PGR and the 3 critical amino acids of AF1 in the mouse PGR. B CRISPR-Cas9-mediated gene editing strategy for generating the mouse line with triple mutant Pgr. The long blue bar indicates Exon 1 of the Pgr gene. The AF1_FFF mouse was generated by pronuclei injection of Cas9 mRNA, 2 Pgr gRNAs, and the homologous single-stranded oligonucleotide (OLN) with the mutations. C AF1_FFF mice exhibit impaired embryo implantation on GD4.5. D The AF1_FFF foetuses develop poorly on GD7.5. E The AF1_FFF uterus shows the failure of decidualization in pseudopregnant mice