Fig. 4

The GD4.5 AF1_FFF uterus also exhibits transcriptome of heightened oestrogenic response. A Top 10 hallmark gene sets enriched in the AF1_FFF (blue) and WT (red bars) uteri. The numbers next to the bars are FDR q values for each gene set. NES, normalized enrichment score. B Venn diagrams indicating the number of genes commonly regulated by EB in OVX mice and dysregulated in the AF1_FFF (AF1M) uterus (padj < 0.05). C, D Heatmaps indicating that approximately 75% up- and downregulated genes by EB (padj < 0.05) are also correspondingly up- or downregulated in the AF1_FFF (AF1M) on GD4.5. In C, genes above the dotted line are commonly upregulated. In D, genes within the dotted rectangle are commonly downregulated (n = 3 for OVX WT mice; n = 4 for GD4.5 mice)