Table 1 Summary of the CRISPR/Cas9 directed mutations from G0 to G2
| Â | Â | Total number | Mutated number | Germline mutation |
|---|---|---|---|---|
G0 | Injected embryo | 711 | / | / |
| Â | Hatched larvae | 77 | / | / |
| Â | Adults | 48 | 36 (75%) | 13 (36.1%) |
| Â | Â | Wild type | Heterozygotes | Homozygous |
G1 | Adult | 49 | 21 (− 2 bp) | 0 |
|  |  | 5 | 9 (− 5 bp) | 0 |
|  |  | 24 | 17 (− 1, + 4 bp) | 0 |
|  |  | 51 | 8 (− 7 bp) | 0 |
|  |  | 20 | 17 (+ 3 bp) | 0 |
|  |  | 34 | 41 (− 12 bp) | 0 |
|  |  | 23 | 36 (− 2, + 7 bp) | 0 |
|  |  | 12 | 7 (− 4 bp) | 0 |
|  |  | 9 | 8 (− 1, + 5 bp) | 0 |
|  |  | 21 | 19 (− 1 bp) | 0 |
|  |  | 7 | 10 (− 1, + 3 bp) | 0 |
|  |  | 11 | 13 (− 3 bp) | 0 |
|  |  | 33 | 21 (− 8 bp) | 0 |
| Â | Total | 299 (56.84%) | 227 (43.15%) | 0 |
|  |  | Wild type | Heterozygotes | Homozygous (− 2 bp) |
G2 | Adults | 27 (24.54%) | 48 (43.63%) | 35 (31.82%) |