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Fig. 2 | BMC Biology

Fig. 2

From: Dietary-derived vitamin B12 protects Caenorhabditis elegans from thiol-reducing agents

Fig. 2

Survival on DTT is influenced by vitamin B12 availability and is METR-1-dependent. ah Plate images of wild-type C. elegans (N2) on animal-derived peptone NGM agar (ad) or on soy-derived NGM agar (eh). Worms were reared on OP50-1 (a, e), with OP50-1 plus 5 mM DTT (b, f), with HT115(DE3) (c, g), or with HT115(DE3) plus 5 mM DTT (d, h). Wild-type (N2) embryos were added at day 0, and images were taken at day 4. in Development of wild-type C. elegans (N2) embryos (ik) or C. elegans metr-1(ok521) mutant embryos (ln) over 4 days on soybean peptone NGM agar and E. coli strain OP50-1 in the absence of DTT (i, l), in the presence of 5 mM DTT (j, m), or in the presence of 5 mM DTT plus 64 nM vitamin B12 (k, n). Scale bars for all images denote 1 mm. o Plotted percentage development of wild-type (N2) or metr-1 mutant worms to adulthood after 4 days of treatment with no DTT (n = N2: 202, metr-1: 142), 0.5 mM DTT (n = N2: 240, metr-1: 174), 1 mM DTT (n = N2: 292, metr-1: 134), 2 mM DTT (n = N2: 290, metr-1: 162), or 5 mM DTT (n = N2: 228, metr-1: 116). p-values were determined from Fisher’s exact test. NS, not significant, *p < 0.05, ***p < 0.001. Purple significance marks indicate comparison of metr-1 mutant to N2 wild type for each treatment group

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