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Fig. 1 | BMC Biology

Fig. 1

From: orsai, the Drosophila homolog of human ETFRF1, links lipid catabolism to growth control

Fig. 1

A P[UAS] insertional mutant exhibits abnormal food-seeking behavior. The P[UAS]100B insertion triggers developmental arrest and premature death. A Graph shows the size of larvae grown in standard agar plates at 24, 48, and 72h AEL. Homozygous P[UAS]100B larvae barely increase their initial size at hatching and die around 72–80h AEL without any of the morphological correlates of third instar. Individuals were taken from 3 independent agar plates. B–E osi mutants die at first instar (L1). B, C Images correspond to mouth hooks from a control second instar (L2, B) and an osi mutant larva 72h AEL (C). D, E Schematic drawings of mouth hooks from a L2, showing three median teeth (D) and a L1, displaying a single tooth in the middle of the hook (E). F Proportion of animals registered as clear gut larvae representing those that did not feed during the experiment (control, P[UAS]100B+/− and P[UAS]100B). G Percentage of the population outside of the yeast patch, highlighting the aberrant mutant behavior. In F and G, each dot represents an independent determination comprising an initial number of 25 larvae per genotype. H Representative images of 72h larvae in agar plates supplemented with a colored yeast patch (control, heterozygous and homozygous P[UAS]100B). Homozygous P[UAS]100B insertion associates with developmental arrest. I Olfactory responses are spared in mutant larvae, showing no differences between genotypes. Each dot represents the collected olfactory response of 50 larvae. All panels: The total number of animals is expressed in the graph under each data set. Shapiro-Wilk test was used for normality assessment, when normality was confirmed one-way ANOVA with Bonferroni’s multiple comparisons was performed. For nonparametric assessment, Kruskal-Wallis with Dunn’s multiple comparisons test were performed (see Additional file 2). Different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. The total number of samples analyzed is included in the corresponding column (bottom). All datasets and statistical analysis on which the conclusions are based are included in Additional file 2

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