Fig. 7

A key role for osi in the fat body. A osi downregulation in the nervous system using elavG4 or nSybG4 does not affect viability. N=3. B–D osi downregulation in the gut does not affect viability. NP1G4 is expressed in the gut of 72h AEL larvae. B, C show GFP driven by NP1G4 confirming its expression in the gut (B) but not in the fat body (C). N=3. E–M cgGal4 recapitulates osi-related phenotypes. E cgGal4 directed expression of GFP to the fat body. F qPCR shows osi levels are reduced about 50%. N=4; each replica included 20 larvae. G Survival indicates the percentage of living individuals. N=2, one representative experiment is shown. H Lip3 downregulation in the fat body accounts for partial survival. Adult eclosion of the indicated genotypes was quantified. The graph shows the proportion of animals obtained relative to the expected (“Methods”). N=1. I: LYRm5 expression in the fat body partially rescues the lethality associated to reduced osi levels. Adult eclosion was quantified. N=2. J Downregulation of osi and EtfQ0 in the cgG4 pattern progress through development, molt and pupate; even though no viable adult eclosed. A representative experiment (N=2) is included. K wal (EtfA) downregulation in the fat body fully rescues survival with reduced osi levels. N=3. L, M osi downregulation in the fat affects lipid droplet content. L Reactive area of lipid droplets stained with BODIPY was quantified. Six to eight individuals were analyzed per genotype. Each dot represents the mean reactive area per animal. M representative images. All panels: different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. The total number of animals analyzed is indicated in D, H, I, and K. A Shapiro-Wilk test was used for normality assessment. A T-test or one-way ANOVA with Bonferroni’s multiple comparisons was performed (see Additional file 2). The total number of samples analyzed is described below each dataset. All datasets and statistical analysis on which the conclusions are based are included in Additional file 2