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Fig. 4 | BMC Biology

Fig. 4

From: Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Fig. 4

Phylogenetic characterization of Fuchi and other spider GATA family proteins. A Schematic of GATA-type DNA-binding domain. This domain comprises two zinc finger motifs (ZF1 and ZF2) and two basic regions (BR1 and BR2). B Amino acid sequence alignment of the ZF1-BR1-ZF2 region (plus one adjacent residue on the N-terminal side) of three canonical GATA family members from cnidarian, mouse, and Drosophila (Nvec_GATA, Nematostella vectensis GATA; Mmus_GATA6, Mus musculus GATA-6; Dmel_Srp, Drosophila melanogaster Serpent). C Amino acid sequence alignment of the ZF1 (plus one flanking residue on the N-terminal side) of various GATA family members from P. tepidariorum and other spider species. There are five canonical (Ptep_GATA1 to GATA5) and three noncanonical (Ptep_GATAL1, Fuchi, GATAL2b) GATA family members in P. tepidariorum. The GATAL2 group, including Fuchi, is characterized by the signature sequence highlighted in yellow, and the GATA5 group by the signature sequence highlighted in green. There are two types of GATAL2 proteins; the ZF1 of one type is aligned with that of the canonical GATA without gap but the ZF1 of the other type is not. D Classification of spider GATAs based on cysteine residue spacing patterns in the ZF1-BR1-ZF2 region. GATA5s in some spider species had a BR1 of slightly varied length. E Diagram showing phylogeny of spider taxa, based on a recent study [107]. F ML tree of 118 canonical and noncanonical GATA family members from Araneae, non-Araneae chelicerates, non-chelicerate arthropods, non-arthropod bilaterians, and cnidarian, using 75 amino acid sites unambiguously alignable. GATAL2 proteins with imperfectly aligned ZF1, including Fuchi, were excluded from this analysis. Bootstrap values (100 replicates) are presented at selected nodes. Color geometric codes indicate taxonomic groups of the sequence source. Details of species names and sequences are available in Tables S7 and S8 (Additional file 7)

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