Fig. 2

Whole-genome sequencing and untargeted metabolite analyses of 102 Zymoseptoria tritici isolates collected from a single field. A Number of isolates collected from each of the eleven cultivars at each collection time point (C1–3; early, middle and late in the season; see also [44]). B The first two principal components (PC) from a PC analysis of genome-wide SNPs. Isolates are color coded by the collection time point. C The first two PCs from a PC analysis of 2633 metabolite markers. Isolates are color coded by the collection time point. D Cross-validation for the determination of optimum number of PCs to be retained for the discriminant analysis of principal components (DAPC) and DAPC plot. Isolates are color coded by the collection time point. E Bi-plot of 2633 metabolite markers showing the ten metabolite markers contributing most to the differentiation of the metabolite profiles. Metabolite markers are labeled using their respective m/z values