Fig. 2.

RNA-seq suggested that hypoxia regulated mESC differentiation through the Wnt/β-Catenin pathway. A The relative levels of the top 50 differentially expressed genes (DEGs) in AB2.2 mESCs on differentiation day 4 under hypoxia versus normoxia. B The GO terms of the DEGs under hypoxia versus normoxia. C The relative levels of the marker genes of three germ layers in AB2.2 mESCs on differentiation day 4 under hypoxia versus normoxia. D The pathway analysis of the DEGs under hypoxia versus normoxia. E The expression of genes related to the HIF-1 signaling pathway in AB2.2 mESCs on differentiation day 4 under hypoxia versus normoxia. F GSEA showed that HIF-1 signaling pathway activation was enhanced by hypoxia. G The expression of Wnt family genes in AB2.2 mESCs on differentiation day 4 under hypoxia versus normoxia. H The expression of Wnt/β-Catenin pathway-related genes in AB2.2 mESCs on differentiation day 4 under hypoxia versus normoxia. I GSEA showed that the Wnt/β-Catenin pathway was repressed by hypoxia. *, significant (P<0.05)