Fig. 2

Evolutionary relationships of isolates from the constructed pangenome of Pyrenophora tritici-repentis. a Maximum-likelihood phylogenetic tree of Pyrenophora tritici-repentis based on aligned and concatenated proteins present in the core genome (total 10,159 genes). Isolate names are followed by their race (R1 to R8) designation, necrotrophic effector(s) produced (A, B, and/or C), and their location of origin (isolates collected within Canada show province names; isolates from outside Canada show country of origin). Presence of ToxA (green circles) and ToxB (orange circles), confirmed via BLAST, and presence of ToxC (grey circles) determined by phenotyping. b Hierarchical sets of Pyrenophora tritici-repentis accessory genes, set family size represents the number of genes shared by isolates in each group, where inclusion in a group is based on any given isolate overlapping with the horizontal bars