Fig. 3

Induction of photothrombotic stroke leads to permanent focal ischemia in the cortex. A Schematic time course of experimental interventions. B Schematic representation of stroke procedure. C Laser Doppler imaging (LDI) of three representative baseline and stroked brains 24 h after injury. D Quantification of stroke area and stroke volume at 21 dpi. Stroke area: Each dot represents the mean of the corresponding subgroup (red: stroke, grey: intact). E Representative histological overview of cortical damage (Neurons, cyan), inflammatory infiltration (Iba1+, magenta), and scar formation (GFAP⁺, green) at 21 dpi, scale bar: 100 μm. Data are shown as mean distributions where the white dot represents the mean. Boxplots indicate the 25 to 75% quartiles of the data. For boxplots: each dot in the plots represents one animal. Line graphs are plotted as mean ± sem. Significance of mean differences between the groups (baseline hemisphere, contralesional hemisphere, and ipsilesional hemisphere) was assessed using Tukey’s HSD. Asterisks indicate significance: ^∗∗∗P < 0.001. ctx, cortex; cc, corpus callosum; ap, anterior posterior; p.i., post injury; ibz, ischemic border zone