Fig. 6

Fat body heterochromatin levels are downregulated in the early stage but upregulated in the middle stage of P. aeruginosa infection. A Schematic of fly collection at different time points of the early and middle stages of PA14 infection for heterochromatin marker immunostaining. B Representative images of fat body cells from wild type flies with HP1a (red) and H3K9me2 (magenta) immunostaining, and nuclear DNA (blue) staining with Hochest. Scale bar: 10 μm. C, D Immunofluorescence intensity of HP1a (C) and H3K9me2 (D) before infection and at 15, 21, and 27 hpi. All results involved staining of tissue from two flies (n = 15/fly). Data are shown as mean ± SD and *p < 0.05, ***p < 0.001 by Student’s t-test. hpi, hrs post infection; ns, not significant