Fig. 3
Genes encoding secreted, non-enzymatic proteinaceous effector candidates (ECs) of Venturia inaequalis are expressed in temporal waves during colonization of susceptible apple cultivar ‘Royal Gala’. A Heatmap of genes demonstrating peak expression during waves 1 and 2 of the early infection stage at 12 and 24 h post-inoculation (hpi). B Heatmap of genes demonstrating peak expression during wave 3 of the mid infection stage at 2 and 3 days post-inoculation (dpi). C Heatmap of genes demonstrating peak expression during waves 4 and 5 of the mid-late infection stage at 5 and 7 dpi. Heatmap gene expression data are scaled rlog-normalized counts across all samples (Z-score). Bar plots depict the maximum log2 DESeq2-normalized count value across all in planta time points. Black annotations indicate genes encoding proteins that belong to an expanded EC family (≥5 members), with brackets highlighting large families and families with sequence similarity to avirulence (Avr) effector proteins from other plant-pathogenic fungi