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Fig. 3. | BMC Biology

Fig. 3.

From: Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0

Fig. 3.

The metrics and rG4 detection outcomes of benchmarking rG4-seq 2.0 experiments. A Bioinformatic workflow of the pre-processing and deduplication of rG4-seq sequencing data. The estimated loss of sequencing reads in each step is highlighted. B Changes in the relative yield of reads in benchmarking rG4-seq 2.0 libraries through the pre-processing and deduplication steps. C Distribution of RTS sites detected in the benchmarking rG4-seq 2.0 experiments. RTS sites were assigned to rG4 structural classes according to their adjacent nucleotide sequences. RTS sites classified as “Others” were considered false positive detections as their adjacent nucleotide sequences do not satisfy the minimum requirements for forming quadruplex or triplex structures. D Reproducibility of RTS sites within biological replicates of benchmarking rG4-seq experiments. Raw data values are provided in the Additional file 8

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