Fig. 4.
From: Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0
The metrics and rG4 detection outcome of the 100-ng input rG4-seq 1.0 library. A Changes in the relative yield of reads in the 100-ng input rG4-seq 1.0 library through the pre-processing and deduplication steps. B Distribution of RTS sites detected in the 100-ng input rG4-seq 1.0 experiment. RTS sites were assigned to rG4 structural classes according to their adjacent nucleotide sequences. RTS sites classified as “Others” were considered false positive detections as their adjacent nucleotide sequences do not satisfy the minimum requirements for forming quadruplex or triplex structures. C Reproducibility of RTS sites within biological replicates of the 100-ng input rG4-seq 1.0 experiment. D Intersection of consensus RTS sites detected in 500-ng input rG4-seq 2.0 experiment and 100-ng input rG4-seq 1.0 experiment. Raw data values are provided in the Additional file 8