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Fig. 6. | BMC Biology

Fig. 6.

From: Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0

Fig. 6.

Reproducibility of RTS sites across benchmarking rG4-seq 2.0 experiments. The intersection of consensus RTS sites detected in A 500-ng input and 100-ng input libraries, B 500-ng input and 30-ng input libraries, and C 500-ng input and 10-ng input libraries. The Venn diagrams indicate the number of RTS sites that are agreed or non-agreed between the two libraries. The swarm plots and overlayed box plots display and contrast the differences in read coverages of agreed/non-agreed RTS sites. The scatter plots indicate the correlation and linear regression outcomes of read coverage of the RTS sites agreed between two rG4-seq libraries. D–G Average read coverages and relative abundances of the parent gene of the consensus RTS sites detected in the rG4-seq experiments. The contour lines from kernel density estimation were overlayed to help illustrate the density distribution of RTS sites

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