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Fig. 8 | BMC Biology

Fig. 8

From: Temporal change in chromatin accessibility predicts regulators of nodulation in Medicago truncatula

Fig. 8

RNAi knockdown of EIN3 and ERF1 reduced the number of nodules on M. truncatula plants. A Data for empty vector control, and EIN3 and ERF1 knock down roots (n = 20, 16, and 13 replicates respectively) were analyzed by ANOVA followed by Tukey’s HSD test for multiple comparisons. Box plots not connected by the same letter are significantly different (P < 0.05). One extreme outlier (29 nodules) was excluded in the MtrunA17Chrg0186741 (ERF1) experiment. B Images of nodules on subtending root supporting the effectiveness of RNAi. Blue color (top) indicates the rhizobial infection (S. meliloti constitutively expressing lacZ), and the red fluorescence marker (bottom) identifies transgenic roots (white scale bar = 1 mm)

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