Fig. 2

GTE4 tends to bind to active genes. A Chromosomal view of GTE4 occupancy. H3K9ac distribution from published data were also plotted as marker of euchromatin. Left Y-axis represents relative GTE4 enrichment; right Y-axis represents relative enrichment of H3K9ac. Black triangles indicate positions of centromere. B Heatmaps showing distribution patterns of GTE4 protein and H3K9ac on all genes. TSS and TTS represent transcription start site and transcription terminal site, respectively. -2 kb and 2 kb represent 2 kb upstream of TSS and 2 kb downstream of TTS, respectively. C Distribution of GTE4-enriched peaks. “Promoter” indicates 1 kb upstream region from TSS; “TSS + 200 bp” indicates TSS and its 200 bp downstream region; “Gene body-200 bp” indicates gene body excluding “TSS + 200 bp”; the rest of genome is defined as “Intergenic.” D Snapshots of IGV view of GTE4-HA ChIP-seq, H3K9ac ChIP-seq, and RNA-seq data of representative region in chromosome 1. Dashed boxes indicate lowly expressed genes with neither GTE4 binding nor H3K9ac modification. Not all loci number were listed due to space limit. E Metaplots showing correlation between GTE4 enrichment and typical histone modifications. ChIP-seq reads density of each modification was calculated for GTE4 peaks using published data. The summit of GTE4 peaks was set as “0,” -2 kb and 2 kb indicate 2 kb up- and down-stream of “0,” respectively. F Metaplots showing correlation between expression levels and GTE4 enrichment. All genes were divided evenly into 3 groups based on expression level; then, GTE4 enrichment was calculated for each group of genes. TSS, transcription start sites; TTS, transcription terminal sites; -2 kb and 2 kb represent 2 kb upstream of TSS and 2 kb downstream of TSS, respectively