Fig. 7

GTE4 suppresses JA biosynthesis through binding and repressing OPR3. A Brief diagram of JA biosynthesis. LOX, lipoxygenase; AOS, hydroperoxide dehydratase; AOC, allene oxide cyclase; OPDA, 12-oxophyto-10,15-dienoic acid. B Snapshots of IGV view of GTE4-HA ChIP-seq and gte4 RNA-seq data of OPR3 locus. C ChIP-qPCR verification of GTE4-enrichment on OPR3. Data are presented as mean ± SEM of 3 technical replicates. Position of primer is indicated with red lines. D RT-qPCR verification of OPR3 upregulation in gte4. Two biological repeats are presented. Data are presented as mean ± SEM of 3 technical replicates for each biological repeat. E RT-qPCR showing expression of OPR3, JA-responsive genes LOX3 and DABB1, and PR1 expression in OPR3 knockdown in gte4 background plants. RNAi-1 and RNAi-2 indicate two independent lines. Data are presented as mean ± SEM of 2 biological replicates. F Growth population of Pst DC300 in gte4 and 2 independent lines of OPR3 knockdown (KD) in gte4 background plants at 3 dpi. Data are presented as mean ± SEM of at least 3 biological replicates. P value was calculated using Student’s t-test