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Fig. 5 | BMC Biology

Fig. 5

From: ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana

Fig. 5

ERF49 is a positive regulator in the BR pathway. a The phenotype of ERF49 dominant-negative transgenic lines in a bzr1-1D background. Scale bar for the siliques, 5 mm. The rest of scale bar, 2.5 cm. b Analysis of hypocotyl elongation in the ERF49 transgenic plants with 2, 4-eBL or 80% ethanol (as control) treatment. Scale bar, 1 cm. Hypocotyl lengths of over 30 seedlings were measured using ImageJ for each independent line. The standardized data is shown with mock as 100% (lower panel). c Relative expression level of downstream gene CPD regulated by BR in the ERF49 transgenic lines. d Relative expression level of BR-regulated downstream genes XTR6 and SAUR_AC1 in bzr1-1D, det2, and ERF49 dominant-negative transgenic lines. Asterisks represent statistical significance (*, P < 0.05; ***, P < 0.001; ****, P < 0.0001; one-way ANOVA followed by post hoc Tukey’s multiple comparison test). UBC30 was used as a reference gene. Numerical data is provided in Additional file 6. The experiments were repeated three times, error bars indicate standard deviation

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