Fig. 6

The response of dominant-negative ERF49 in Col-0 or bzr1-1D to heat stress. a The relative expression level of ERF49 in eight ERF49 dominant-negative transgenic lines. b, c The phenotype and survival rates of ERF49 dominant-negative transgenic lines in Col-0 or bzr1-1D after heat stress treatment at 42 °C for 3 hours and recovery for 5 days. Sixty to eighty seedlings of each genotype from three biological replicates were counted. Col-0 and bzr1-1D were used as controls. Asterisks represent statistical significance (**, P<0.01; ***, P < 0.001; ****, P < 0.0001; one-way ANOVA followed by post hoc Tukey’s multiple comparison test). Numerical data is provided in Additional file 6. The experiments were repeated three times, error bars indicate standard deviation