Fig. 2

Microfluidic device yields growth and repair dynamics of populations starting from single cells. a Representative images of cells trapped in microfluidic wells at t = 0, 12, and 24 h after galactose induction, for the 8 experimental conditions: NR, CGG, GAA, or CTG target sequences with either Cas9 or Cpf1. The scale bar represents 50 μm. b Corresponding growth curves for individual wells in each condition. Blue lines represent the number of cells per time point and green lines the number of GFP+ cells. Bold lines correspond to the total number of cells and GFP+ cells at each time point, for each experimental condition