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Fig. 5 | BMC Biology

Fig. 5

From: Time-resolved microfluidics unravels individual cellular fates during double-strand break repair

Fig. 5

Dynamics and statistics of individual lineages. A NR-Cas9. a Dynamics of \(N^\text{local}_\text{tot}\) (blue) and \(N^\text{local}_\text{GFP}\) (green) from six randomly selected wells. Note the diverse dynamics from different positions. b Distribution of delay times between first division and first detection of GFP+ cell. c Local fraction \(R^{\text {local}}=N^\text{local}_\text{GFP}/N^\text{local}_\text{tot}\) for 9 randomly selected positions. The transitions from \(R^\text{local}=0\) happen at different instants and quickly rise towards \(R^\text {local}\simeq 1\). Note that in some cases \(R^\text{local}\) remains zero. d Distribution of values of \(R^\text {local}\) at the end of the experiment. e Distribution of \(R^{\text {local}}\) at the end of the simulation obtained from the stochastic model using the same parameter values as above. B CTG-Cpf1. C CTG-Cas9. Same graphs as above

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