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Fig. 4 | BMC Biology

Fig. 4

From: A bacteria-regulated gut peptide determines host dependence on specific bacteria to support host juvenile development and survival

Fig. 4

Increased expression of Imp-L2 in the GF enterocytes is reversed by Ap re-association but not by Lp. a Transcript levels of Imp-L2 in the gut significantly increase after subjecting flies to GF conditions, whereas those in the fat body or in the body wall muscle do not. b Mono-association of Lp (WJL) fails to decrease the transcript level of Imp-L2 in the gut, whereas mono-association of Ap (DM001) significantly decreases this level. Mono-association of P3G5 decreases this level in a manner similar to that shown by Ap (DM001). c Immunohistochemical analysis of Imp-L2 expression in cells of the larval gut. The immune signals of Imp-L2 (green) in midgut enterocytes are manifested in GF, and this phenomenon is observed in Lp (WJL) mono-association. These immune signals disappear in Ap (DM001) or P3G5 mono-association. 4′,6-Diamidino-2-phenylindole (DAPI) staining (blue) shows nuclei. d Quantification of Imp-L2 immune signals in gut enterocytes shown in c. *p < 0.05 and ***p < 0.001 compared to values obtained for GF larvae (t-test). n.s., not statistically significant. In a and b, error bars denote standard error of the mean (SEM) and values shown are obtained from five independent observations. In d, bars in the graph represent mean and SEM, and each dot shows the value from each independent replicate

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