Fig. 5

Lp and Ap differentially impact Imp-L2-mediated FOXO regulation. a, b Mono-association of either Lp (WJL) or Ap (DM001) increases the activity of PI3K, estimated by GFP intensity of tGPH, in gut enterocytes (a) and in fat body cells (b) of wild type larvae. Mutation of Imp-L2 increases PI3K activities both in enterocytes (a) and in fat body cells (b) in GF, which are not further increased by association of either Lp (WJL) or Ap (DM001). c Mono-association of either Lp (WJL) or Ap (DM001) increases the activity of Akt in fat body, verified using western blot for phosphorylated-Akt. Mutation of Imp-L2 increases Akt activities in GF fat body cells, which are not further increased by association of either Lp (WJL) or Ap (DM001). d–f Mutation of Imp-L2 fails to remove FOXO from the nucleus to the cytoplasm of both enterocytes (d) and fat body cells (e) in GF. Mono-association of Ap (DM001) strongly removes FOXO from the nucleus to the cytoplasm, whereas that of Lp (WJL) partially does. The quantifications of FOXO localization in enterocytes and fat cells are shown in the lower panel of d and e, respectively. f The mRNA level of 4E-BP, a target gene of FOXO, does not decrease after Imp-L2 mutation in GF. Mono-association of Ap (DM001) strongly decreases mRNA level of 4E-BP, whereas that of Lp (WJL) partially does. In a, b, d, and e, DAPI staining is also seen in nucleus. * p < 0.05 (t-test). n.s., not statistically significant. Error bars denote SEM