Fig. 4

Regulatory elements (REs) drive interspecies transcriptional conservation during evolution. A Comparison of total branch lengths for the phylogenetic trees across four categories of one-to-one orthologues genes based on the 1000 bootstrapping test. Enhancer, Promoter, Both, and None indicate genes with only enhancers, only promoters, both enhancers, and promoters, without REs, respectively. Box plots showing the median phastCons scores (B) and pLI score (C) for four categories of one-to-one orthologues genes. D The number of associated enhancers and promoters contributes to interspecies transcriptional stability. E The association of gene stability and conserved REs, including highly conserved enhancers (ACE) and highly conserved promoters (ACP). “**” indicates P < 0.01; “***” indicates P < 0.001; “****” indicates P < 0.0001