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Fig. 1. | BMC Biology

Fig. 1.

From: AHL-Priming Protein 1 mediates N-3-oxo-tetradecanoyl-homoserine lactone priming in Arabidopsis

Fig. 1.

Principal mechanism of AHL-induced priming is missing in ali1. a Expression analysis of two AHL-priming marker genes (TLP5 and DFR) in Col-0 and ali1 72 h after priming with 6 µM oxo-C14-HSL. The abundance of each gene transcript was normalized with the Ubiquitin ligase (At5g25760) transcript and acetone control. The bar represents mean and error bars represent SD from eight independent biological repetitions from three experimental replicates. b Phosphorylation pattern of the AtMPK6 in response to flg22 challenge in Col-0 and ali1, pretreated with 6 µM oxo-C14-HSL or acetone (control). Western blot analysis was performed in three independent experiments, representative blots are shown. c Representative images of Col-0 wild-type and the ali1 mutant. d The expression profile of defense-related genes WRKY22 and WRKY29 was monitored at various time points after 100 nM flg22 challenge in Col-0 and ali1 mutant. The abundance of each gene transcript was normalized with the Ubiquitin ligase (At5g25760) transcript and 0 hpt (hours post treatment) levels. Error bars represent SD from four independent biological repetitions. Experiments were carried out three times with similar results. In a and d, data are presented as mean ± SD. * P ≤ 0.05 (Student’s t test), individual data values for a and d in Additional File 1

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