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Fig. 6. | BMC Biology

Fig. 6.

From: AHL-Priming Protein 1 mediates N-3-oxo-tetradecanoyl-homoserine lactone priming in Arabidopsis

Fig. 6.

AHL-induced priming is restored in complemented ali1. The expression profile of three defense-related genes a WRKY22, b WRKY29, and c GST6 were monitored before (0 h) and after (2 h) 100 nM flg22 challenge in two complemented lines of ali1: #10–2 and #10–19 as well as an outcross (non-complemented) line #10–3 that were earlier primed with 6 µM oxo-C14-HSL for 3 days. The abundance of each gene transcript was normalized with the Ubiquitin ligase (At5g25760) transcript and acetone control at 0 hpt before flg22 elicitation. The bar represents the mean and error bar represents SD of three replicates. Experiments were carried out two times with similar results. d Pathogenicity assays with Pst on wild-type Col-0, two complemented lines of ali1: #10–2 and #10–19 as well as an outcross (non-complemented) line #10–3. Plants grown for 3 weeks on ½-strength MS containing acetone or 6 µM oxo-C14-HSL and then challenged with Pst. The bar represents the mean and error bar represents SD of three replicates. Experiments were carried out two times with similar results. e Representative photographs of 6-week-old #10–2, #10–19, and #10–3 plants. In a–d, data are presented as mean ± SD. * P ≤ 0.05 (Student’s t test), individual data values for a–d in Additional File 1

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