Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: Female factors modulate Sex Peptide’s association with sperm in Drosophila melanogaster

Fig. 1

Amount of SP detected on sperm gradually increases from male ejaculate to female bursa and is highest in seminal receptacles. Pre-mating ejaculate samples were collected from Fru>dTRPA1 males exposed to high temperatures, as described in “Methods”. Post-mating sperm samples were isolated from wild type (CS) females that had mated to wildtype (CS) males and were frozen at 35 min and 2 h ASM. Sperm heads were stained with DAPI (blue) and anti-SP staining was visualized with Alexa fluor 488, staining the sperm tail (green) and sperm head (cyan; overlapping blue/green); Bar = 20μm. A, A’ Sperm isolated from ejaculate expelled by Fru>dTRPA1 males. C, C’ Sperm isolated from bursa of the wildtype mated female, frozen at 35 min ASM. E, E’ Sperm isolated from the seminal receptacle of the wildtype mated female, frozen at 2 h ASM. G, G’ Sperm isolated from Fru>dTRPA1 male’s ejaculate that was kept incubated for 2 h in 1× PBS, after exudation. B, D, F, and H are negative controls for A, C, E, and G panels, with only secondary antibody (anti- rabbit, Alexa fluor 488) and no primary antibody (anti-SP) incubation. A’, C’, E’, and G’ panels have an additional transmitted light channel overlay to highlight the outline of sperm tail specifically in samples where the staining was very weak (ejaculate). I Schematic of the time and nature of sample collection. J Corrected fluorescence (CF) intensity of SP on sperm at three different stages and time points. Error bars show Mean±SE (AU stands for arbitrary units); p***<0.001, p**<0.01, ns=not significant, degree of freedom, F(3, 48)= 132; n=13

Back to article page