Fig. 2
From: Lentiviral in situ targeting of stem cells in unperturbed intestinal epithelium
90-days-old ApcMin/+ and Apc+/+ mice were lentivirally targeted on E8.0 with p53 or luciferase shRNA. a, b Intestinal polyp numbers in mice targeted with the indicated shRNAs. Two shRNAs were used for p53 knockdown (sh1558 in black and sh852 in red). a The indicated regions of the intestine were analysed side by side with Welch’s t-test without multiple comparison testing. b The total polyp number in the whole intestine was determined and analysed by Welch’s t-test. a, b sh-luci and sh-p53 polyp numbers in the different intestinal regions were pooled for normality testing and were found to be normally distributed (D’Agostino and Pearson normality test). ApcMin/+ mice: luciferase n = 5, p53 sh842 n = 6, p53 sh1558 n = 4; Apc+/+ mice: luciferase n = 8, H2B-RFP n = 3, p53 sh842 n = 5, p53 sh1558 n = 5. c Mean transduced crypt field area of entire small intestine (duodenum, jejunum and ileum regions combined) transduced with sh-p53 or control sh-luciferase. Apc+/+ regions: sh-luciferase n = 18, sh-p53 n = 21; ApcMin/+ regions: sh-luciferase n = 13, sh-p53 n = 21. d The transduced area of the entire small intestine from WT or ApcMin/+ mice transduced with sh-p53 or control sh-luciferase. Apc+/+ regions: sh-luciferase n = 19, sh-p53 n = 21; ApcMin/+ regions: sh-luciferase n = 13, sh-p53 n = 21. e Number of transduced crypt fields present in the entire small intestine from WT or ApcMin/+ mice transduced with p53 or control luciferase shRNA. No significant differences were found. Apc+/+ regions: sh-luciferase n = 26, sh-p53 n = 27; ApcMin/+ regions: sh-luciferase n = 14; ApcMin/+ sh-p53 n = 29. f Mean average crypt number per field present in the entire small intestine. Apc+/+ regions: sh-luciferase n = 22, sh-p53 n = 22; ApcMin/+ regions: sh-luciferase n = 12, sh-p53 n = 22. g Mean average crypt area present in the whole small intestine. Apc+/+ regions: sh-luciferase n = 15, sh-p53 n = 18; ApcMin/+ mice: sh-luciferase n = 14, sh-p53 n = 18. c–g Data were log2 transformed and analysed with two-way ANOVA and Tukey’s multiple comparisons test—untransformed data plotted