Fig. 2

Histone modification positioning and number as a function of transcription. A Average peak width of different histone modifications (H3K27me3, H3K36me3, H3K4me3, H3K27ac, and H3K9ac) from promoter and gene body as a function of gene expression category in symbiosis. Magenta boxplots are from promoters and pinks are from gene bodies. X-axis is the gene category based on expression level (< 30th percentile RPKM: low, 30th and 70th percentile RPKM: intermediate and > 70th percentile RPKM: high); y-axis log value of peaks’ breadth (unpaired two-tailed Student’s t test; ****p < 0.0001). B Boxplot showing average expression for genes without peaks and genes with peaks for specific histone modifications (unpaired two-tailed Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). C Specific histone modification counts are represented on the y-axis. X-axis represents gene expression values binned in deciles according to mRNA abundance (RPKM). Dashed lines represent the average count of each histone modifications and mCpG for all the genes, also shown in μ. Different chromatin modifications are represented by colors. D Boxplot showing average expression for the genes with two, three, four, and five peaks. And correspondingly with and without the repressive modification H3K27me3 (unpaired two-tailed Student’s t test; ***p < 0.001, ****p < 0.0001)