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Fig. 4 | BMC Biology

Fig. 4

From: Long-term imaging reveals behavioral plasticity during C. elegans dauer exit

Fig. 4

Transcriptome profiling reveals dynamic neuropeptide signaling during dauer exit. a Schematic summarizing the two main behavioral switches identified by behavioral imaging. b Schematic of the head-specific low-input mRNA sequencing method. For each time point and each condition, 10 heads were sequenced. c PCA of all samples, using the log transformed and normalized counts of the 500 most variable genes as input. d Normalized counts of daf-28 across both conditions and all time points. Depicted is the mean ± standard deviation. e Dotplot depicting the result of the GO-term analysis. For each time point, differentially expressed genes between the two conditions were determined, and the result was used for GO-term analysis. Size of the dot scales with gene ratio. Gene ratio refers to the proportion of genes in the input list that are annotated to that GO-term. Shown are the top 3 GO-terms for each time point, and p-values are indicated next to each dot. f Heatmap of genes contained within the “neuropeptide signaling pathway” GO-term, comparing both conditions at 6 h.a.t. One column shows one experimental repeat

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