Fig. 4

Single-cell chromatin accessibility dynamics in response to heat stress. a UMAP visualization of normal and HS scATAC clusters after alignment. Each dot indicates a single cell. The colours indicate normal or HS. b UMAP visualization of separated normal and HS samples of scATAC-seq clusters after alignment. Each dot indicates a single cell. c Bar plot comparison of cell percentage between normal and HS for each cluster. d Scatter plots of GO enrichment analysis of differentially accessible genes for each cluster between normal and HS. Overrepresentation analysis and visualization performed using the ClusterProfiler R package. e, f Genome browser screenshot comparison of chromatin accessibility between normal and HS samples from bulk ATAC-seq (f) and scATAC-seq with each cluster (e)