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Fig. 3 | BMC Biology

Fig. 3

From: A simple high-throughput method for automated detection of Drosophila melanogaster light-dependent behaviours

Fig. 3

Effects of repeated sudden darkness presentation. For 2 h, flies underwent stimuli presentation with a 5-min (upper panels) or 1-min (bottom panels) intra-trial interval (ITI). For analysis, we grouped and averaged the trialsā€™ delta outcomes in 30-min recording epochs, containing n = 6 and n = 30 delta values each for 5 and 1, respectively (right panels). A CSORC wild-type flies showed no reduction of movements with 5-min ITI (p = 0.779, slope = 0.042, R2 = 0.060) but exhibit a significant linear trend with the 1-min ITI protocol (p = 0.0003, slope = āˆ’ā€‰0.153, R2 = 0.932). B The w1118 line showed a significant reduction trend for 5-min ITI (p = 0.041, slope = āˆ’ā€‰0.307, R2 = 0.674), while no difference was found between trials for the 1-min ITI protocol (p = 0.522, slope = 0.039, R2 = 0.338). All analyses were performed on 30-min epoch average values using one-way ANOVA and post hoc test for linear trend (n = 96/group from 3 independent experiments)

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